The anova assumes that the measurement variable, glycogen content, is normal (the distribution fits the bell-shaped normal curve) and homoscedastic (the variances in glycogen content of the different ...The anova assumes that the measurement variable, glycogen content, is normal (the distribution fits the bell-shaped normal curve) and homoscedastic (the variances in glycogen content of the different PGM sequences are equal), and inspecting histograms of the data shows that the data fit these assumptions.