Many statistical tests have been developed to estimate the sample size needed to detect a particular effect, or to estimate the size of the effect that can be detected with a particular sample size. ...Many statistical tests have been developed to estimate the sample size needed to detect a particular effect, or to estimate the size of the effect that can be detected with a particular sample size. In order to do a power analysis, you need to specify an effect size. This is the size of the difference between your null hypothesis and the alternative hypothesis that you hope to detect. For applied and clinical biological research, there may be a very definite effect size that you want to detect.